KIM KAH HWI,LIM KOK HONG,CHEAH SWEE HUNG,POON CHI TAT,POH TING FUNG,LEE SUI TING,JACY MOINON
申请号：
MYPI 20091246
公开号：
MY150716A
申请日:
2009.03.27
申请国别(地区):
MY
年份:
2014
代理人:
摘要：
A COMPOSITION FOR TREATMENT OF LEUKEMIA COMPRISING PHARMACEUTICALLY EFFECTIVE AMOUNT OF AN ACTIVE EXTRACT OF RODGERSIA SAMBUCIFOLIA CHARACTERIZED IN THAT THE ACTIVE EXTRACT IS OBTAINED THROUGH EXTRACTING DRIED GROUND LEAVES OF RODGERSIA SAMBUCIFOLIA WITH A FIRST NON-POLAR AND/OR SEMI-POLAR SOLVENT ACQUIRING A FIRST CRUDE EXTRACT BY CONCENTRATING THE FIRST NON-POLAR AND/OR SEMI-POLAR SOLVENT UPON REMOVING THE EXTRACTED LEAVES DISSOLVING THE FIRST CRUDE EXTRACT IN A MIXTURE OF IMMISCIBLE SECOND POLAR AND SECOND SEMI-POLAR OR NON-POLAR SOLVENT OBTAINING AND CONCENTRATING SECOND SEMI-POLAR OR NON-POLAR SOLVENT TO ACQUIRE A SECOND CRUDE EXTRACT, FRACTIONATING THE SECOND CRUDE EXTRACT USING VACUUM LIQUID CHROMATOGRAPHY WHICH SOLVENT MIXTURE OF CHLOROFORM AND METHANOL IS USED AS A MOBILE PHASE STARTING WITH 100% CHLOROFORM WITH STEPWISE INCREMENT OF METHANOL IN 20% AT EACH STEP AND COLLECTING THE ACTIVE EXTRACT IN FRACTION OF 60% AND/OR 80% METHANOL, WHEREIN SILICA GEL IS USED AN INERT PHASE IN THE VACUUM LIQUID CHROMATOGRAPHY. (MOST ILLUSTRATED BY FIGURE 3)
