A probiotic embedding particle and manufacturing method thereof are disclosed. The disclosed probiotic embedding particle comprises a probiotic sclerotium, a colloidal layer and a fungal hypha shell. The probiotic sclerotium is enclosed by the colloidal layer, and the colloidal layer is enclosed by the fungal hypha shell. The fungal hypha shell and the colloidal layer are functioned as an embedding structure that can effectively protect the probiotics. The fungal hypha shell comprises fungal nutrient substances which are capable of providing human body with health care effect when they are taken. Besides, with the structure of the fungal hypha shell, the stabilities of secondary processing and storage of the disclosed probiotic embedding particle can be increased. Further, the probiotic sclerotium can provide efficacies of body constitution adjustment, digestive functionality maintenance and gut microbiota variation, and the disclosed probiotic embedding particle can have a low manufacturing cost.一種益生菌包埋顆粒,該益生菌包埋顆粒包含一益生菌核、一膠狀物質層以及一真菌菌絲殼層,該膠狀物質層包覆該益生菌核,而該真菌菌絲殼層包覆該膠狀物質層。利用該真菌菌絲殼層和該膠狀物質層作為包埋結構,可有效保護益生菌,且該真菌菌絲殼層係含有真菌營養物質,當人體食入後,可提供保健之效果,再者,藉由該真菌菌絲殼層的結構,可增加該益生菌包埋顆粒二次加工之穩定性及儲存安定性,此外,該益生菌核具有調整體質、維持消化道機能及改變腸道菌相等功效,且該益生菌包埋顆粒的製造成本較低。10‧‧‧益生菌核11‧‧‧菌穩定劑12‧‧‧益生菌20‧‧‧膠狀物質層30‧‧‧真菌菌絲殼層