Imaging systems and methods of using the same are provided for monitoring the quantity of fluorescent pigment, for example lipofuscin, in the retinal pigment epithelium (RPE) layer of a retina in vivo. Various imaging modalities can be integrated into a single system and excited by a single broadband light source for the monitoring of the fluorescent pigment. The influence of varying optical properties found in the optical path of the pigments auto-fluorescence between the RPE and an image receiver can be corrected.
