ФЕДЕРАЛЬНОЕ ГОСУДАРСТВЕННОЕ БЮДЖЕТНОЕ НАУЧНОЕ УЧРЕЖДЕНИЕ "НАУЧНО-ИССЛЕДОВАТЕЛЬСКИЙ ИНСТИ;FEDERAL STATE BUDGETARY INSTITUTION RESEARCH INSTITUTE FOR COMPLEX ISSUES OF CARDIOVASCULAR DISEASES(NII KPSSZ)
发明人:
ANTONOVA, Larisa Valerievna,АНТОНОВА, Лариса Валериевна,KUDRYAVTSEVA, Yuliya Aleksandrovna,КУДРЯВЦЕВА, Юлия Александровна,BARBARASH, Leonid Semenovich,БАРБАРАШ, Леонид Семенович,BARBARASH, Olga Leonid
申请号：
RURU2017/000315
公开号：
WO2017/217887A1
申请日:
2017.05.17
申请国别(地区):
RU
年份:
2017
代理人:
摘要：
The invention relates to the field of medicine and tissue engineering, and may be used in cardiovascular surgery when performing reconstructive vascular surgery. Proposed is a tissue-engineering biodegradable vascular implant made of biodegradable polymers via an electrospinning method, with a layer-by-layer introduction, into a vascular wall, of biologically active molecules such as vascular endothelial growth factor (VEGF), fibroblast growth factor (bFGF) and a chemoattractant molecule (SDF-1α). A polymer composition consists in a mixture of PGBV/PCL at a ratio of 1:2, and is dissolved in chloroform to a concentration of 5% PHBV and 10% PCL. A biodegradable graft is obtained by mixing the polymer solution with a water fraction of differentiation molecules to a final concentration of the latter of between 12.5 and 500 ng per 1 ml of solution. Electrospinning is performed, during 1/3 of the total time for electroforming a graft, using a formulation containing the polymer composition with VEGF molecules, and, during 2/3 of the time, using the polymer solution with a water fraction of the biologically active molecules bFGF and SDF-1a. A tissue-engineering graft prepared in this way corresponds to the layered structure of the vascular wall of a living organism, and the vitality and functional activity of the biologically active components is maintained until the degradation term of the polymer matrix is reached.L'invention se rapporte au domaine de la médecine et du génie tissulaire, et peut être utilisée en chirurgie cardiovasculaire lors d'opérations de reconstruction sur des vaisseaux. L'invention concerne un implant vasculaire biodégradable obtenu par génie tissulaire qui est fait de polymères biodégradables selon un procédé d'électro-filage avec introduction par couches dans la paroi du vaisseau de molécules bio-actives, comme un facteur de croissance endothéliale vsculaire (VEGF), un facteur de croissance de fibroblastes (bFGF) ou une molécule chimioattractive (S
