Johannes Auer,Martin Bader,Stefan Dengl,Stefan Lorenz,Stefan Seeber
申请号：
US14611566
公开号：
US20150218250A1
申请日:
2015.02.02
申请国别(地区):
US
年份:
2015
代理人:
摘要：
Herein is reported a method for the production of a polypeptide that is biologically active as n-mer comprising a nucleic acid encoding a fusion polypeptide according to the following formula (Bn—CSo—Is—CSp—FC—CSq—It—CSr—Bm)u, wherein B denotes a polypeptide that is biologically active as n-mer and forms non-defined aggregates/multimers upon expression in the absence of a fused Fc-region, FC denotes a heavy chain Fc-region polypeptide, CS denotes a cleavage site, and I denotes an intervening amino acid sequence, wherein FC does not substantially bind to an Fc-receptor, recovering the fusion polypeptide from the cell or the cultivation medium, optionally cleaving the fusion polypeptide with a protease, and thereby producing a polypeptide that is biologically active as n-mer and forms non-defined aggregates/multimers upon expression in the absence of a fused Fc-region.
