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intact minizellen as vectors for dna transfer and gene therapy in vitro and in vivo
专利权人:
ENGENEIC MOLECULAR DELIVERY PTY LTD.
发明人:
BRAHMBHATT, HIMANSHU,MACDIARMID, JENNIFER
申请号:
AT02783361
公开号:
AT541940T
申请日:
2002.10.15
申请国别(地区):
AT
年份:
2012
代理人:
摘要:
A composition comprising recombinant, intact minicells that contain a therapeutic nucleic acid molecule is disclosed. Methods for purifying a preparation of such minicells also are disclosed. Additionally, a genetic transformation method is disclosed, which comprises (i) making recombinant, intact minicells available that contain a plasmid comprised of a first nucleic acid segment, and (ii) bringing the minicells into contact with mammalian cells that are engulfing-competent, such that the minicells are engulfed by the mammalian cells, which thereafter produce an expression product of the first nucleic acid segment.
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