PROBLEM TO BE SOLVED: To provide a buckwheat allergen specific monoclonal antibody that can be applied for detection of a variety of buckwheat strains and avoids cross-reaction with seed plants other than buckwheat, and provide a buckwheat allergen specific monoclonal antibody which can detect buckwheat allergens included in not-heated raw materials and food products as well as buckwheat allergens in food products after heat denaturation.SOLUTION: The monoclonal antibody recognizes an amino acid sequence included in a specific region of BWp16 as an epitope.