Agricultural Genomics Institute, Chinese Academy of Agricultural Sciences;Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences
发明人:
申请号：
EP19819225.4
公开号：
EP3718396A1
申请日:
2019.04.11
申请国别(地区):
EP
年份:
2020
代理人:
摘要：
Disclosed is a method for breeding self-compatible potatoes, which comprises the following steps: (1) selecting a self-compatible potato variety material and referring to it as PG6359, and cloning the S-RNase gene of PG6359 through the transcriptome sequencing method； and (2) obtaining two full-length sequences of the S-RNase gene from the cloned S-RNase gene in step (1) and referring to them as Ss11 and Ss12 respectively, and after carrying out an artificial self-pollination for the variety material PG6359, selecting the variety material having the genotype of Ss11Ss11 from the offspring as the female parent and referring to it as material A, and selecting a self-incompatible material as the male parent and referring to it as material B, and then obtaining a self-compatible F1 generation by hybridization. The advantage of the present invention lies in that self-compatible diploid potatoes are generated. The present invention does not require the introduction of any wild potato gene fragments, thereby avoiding linkage drag, and providing a basis for the rapid creation of a diploid potato inbred line.
