VOGELS, RONALD,HAVENGA, MENZO JANS EMCO,ZUIJDGEEST, DAVID ADRIANUS THEODORUS
申请号：
CA2478508
公开号：
CA2478508C
申请日:
2003.04.24
申请国别(地区):
CA
年份:
2013
代理人:
摘要：
The present invention provides methods and means to increase the stabilityand/or the packaging capacity of recombinant adenoviruses, by overexpressionof pIX in an adenoviral packaging cell, by retaining at least a part of theE1B 55K region in the recombinant adenoviral vector or by regulating pIX witha heterologous promoter. The invention further relates to methods and meansfor the production of such adenoviruses on complementing cell lines, whereinthe early region 4 open reading frame 6 (E4-orf6) encoding nucleic acid ispresent in the adenovirus and wherein the E4-orf6 gene product is compatiblewith one or more products of the E1 gene products in the complementing cell,such that the adenoviral vector can be efficiently produced by thecomplementing cell.
