PROBLEM TO BE SOLVED: To provide a hot-water disinfection method capable of improving the high temperature resistance of seeds and efficiently disinfecting the seeds with hot water.SOLUTION: A method of hot-water disinfection of seeds includes: a dehydration treatment process in which moisture content of the seeds is reduced and a hot-water treatment process in which the seeds with reduced moisture content are immersed in hot water. In the dehydration treatment process the moisture content of the seeds is reduced by applying heat, and in the hot-water treatment process the seeds whose grain temperature has increased as a result of the heating are immersed in hot water.