ISOLATED NUCLEIC ACID MOLECULE, GENE CONSTRUCTION, VECTOR, TRANSGENIC CELL, METHOD FOR OBTAINING A CELL AND A TRANSGENIC PLANT, BIODEGRADABLE PESTICIDAL COMPOSITION, A PESTICIDAL LABORATORY METHOD,
EMBRAPA - EMPRESA BRASILEIRA DE PESQUISA AGROPECUÁRIA
发明人:
GUSTAVO RAMOS DE OLIVEIRA,MARIA CRISTINA MATTAR DA SILVA,MARIANA TORQUATO QUEZADO DE MAGALHÃES,Maria Fátima Grossi de Sa,THALES LIMA ROCHA
申请号：
BRPI0906128
公开号：
BRPI0906128B1
申请日:
2009.07.24
申请国别(地区):
BR
年份:
2020
代理人:
摘要：
isolated nucleic acid molecule, gene construction, vector, transgenic cell, method for obtaining a transgenic cell and plant, isolated and purified polypeptide, biodegradable pesticidal composition, method for pest control, method for obtaining resistant transgenic strains to a pest insect. the present invention belongs to a new insecticidal compound derived from a lineage of bacilius thuringiensis and refers to the field of plant pest control, particularly to the control of the cotton weevil - anthonomus grandis. more specifically, the object of the invention relates to a new gene for a new delta-endotoxin called cry8ha and the cloning and expression in escherichia coli of the gene encoding the cry8ha protein. the nucleotide and protein sequence encoding the new delta-endotoxin, recombinant vectors and host cells are provided. processes and means are also provided for recombinant production and the use of the new delta-endotoxin for application in the control of the cotton weevil. in addition, the invention also provides a synthetic gene optimized for expression in cotton plants. using the gene described here, it is possible to transform plants, using techniques known to experts in the field, for the expression of the active endotoxin against the cotton weevil.molécula de ácido nucléico isolada, construção gênica, vetor, célula transgênica, método para obtenção de uma célula e de uma planta transgênica, polipeptídeo isolado e purificado, composição pesticida biodegradável, método para o controle de uma praga, método de obtenção de linhagens transgênicas resistentes a um inseto praga.a presente invenção pertence a um novo composto inseticida derivado de uma linhagem de bacilius thuringiensis e refere-se ao campo de controle de pragas de plantas, particularmente ao controle do bicudo-do-algodoeiro - anthonomus grandis. mais especificamente, o objeto da invenção refere-se a um novo gene para uma nova delta-endotoxina designada cry8ha e a clonagem e expressão em escher
