INSTITUT NATIONAL DE LA SANTÉ ET DE LA RECHERCHE MÉDICALE (INSERM);TXCELL S.A.
发明人:
申请号：
EP06808973.9
公开号：
EP1899459B1
申请日:
2006.07.03
申请国别(地区):
EP
年份:
2016
代理人:
摘要：
The invention relates to an in vitro method for the obtention of a food- or auto-antigen specific Trl cell population from a leukocyte or a PBMC population, which comprises stimulating the PBMC or leukocyte population with the food- or auto-antigen, and recovering the food- or auto-antigen specific Trl cell population from the stimulated cell population. Preferably, the PBMC or leukocyte population is re-stimulated at least once with the same antigen after step (1), in the presence of IL-2 and at least one interleukin selected from the group consisting of IL-4 and IL-13.The in vitro method may further comprise a third step of expanding the recovered antigen-specific Trl cell population, advantageously by contacting them with feeder cells capable of expressing factors necessary for said expansion. Preferably, the feeder cells are recombinant insect feeder cells.
