The present invention relates to a tissue culture process for the production of Indian genotypes of date palm (Phoenix dactylifera L.) which comprises of explant preparation, surface sterilization of explant, establishment of explant, callus induction, callus multiplication, induction of somatic embryos, multiplication of somatic embryos, maturation of somatic embryos, germination of somatic embryos giving rise to root and shoot, in vitro plantlet growth and hardening. It provides a tissue culture process for the multiplication of Indian genotypes of date palm having least chances of somaclonal variation and a standardized process for the hardening of tissue culture raised plantlets.
