PROBLEM TO BE SOLVED: To provide lactic acid bacteria with enhanced immunostimulating activity not mixed with esters of polyhydric alcohols and fatty acids.SOLUTION: The invention provides a method comprising a step of contacting a lactic acid bacterium having an immunostimulating activity with a composition containing an ester of a polyhydric alcohol and a fatty acid as an effective ingredient to enhance the immunostimulating activity of the lactic acid bacterium; and then a step of washing the lactic acid bacterium to remove the composition to produce a lactic acid bacterium having an enhanced immunostimulating activity but free from the ester of the polyhydric alcohol and the fatty acid.SELECTED DRAWING: None【課題】多価アルコールと脂肪酸のエステル結合物が混合されていない状態の、免疫賦活作用が増強された乳酸菌の提供。【解決手段】多価アルコールと脂肪酸のエステル結合物を有効成分として含む乳酸菌免疫賦活作用増強組成物と免疫賦活作用を有する乳酸菌とを接触させ、その後乳酸菌を洗浄し、前記乳酸菌免疫賦活作用増強組成物を取り除くことを含む、免疫賦活作用が増強され多価アルコールと脂肪酸のエステル結合物を含まない乳酸菌を製造する方法。【選択図】なし