The present invention provides a fully human anti-VEGF monoclonal antibody,the preparationmethod and use thereof. The fully human anti-VEGF monoclonal antibody isobtained by usingantibody phage display technology, which has higher antibody affinity andstronger capacity forinhibiting tumor cell proliferation in comparison with humanized antibodybevacizumab, andcan be used to prepare anti-tumor medicines.