The present invention relates to killed lactic acid bacteria preparation having enhanced immunity and a method for preparing the same, and more particularly, relates to killed lactic acid bacteria preparation having enhanced immunity and a method for preparing the same, the method comprising the steps of culturing lactic acid bacteria in a culture medium containing surfactant and carbonate and killing. According to the present invention, it is possible to produce lactic acid bacteria preparation that has enhanced immunity effect, as well as, the effect of preventing functional damage of surface-expressed target protein, with high concentration rate. Since the killed lactic acid bacteria prepared according to the present invention shows improved immunity enhancing effect and enables mass production compared to live bacteria, it is useful as feed additives, animal drugs or vaccines etc.
