A method of creating an implantation material from biological and tissueengineered biosynthetic and biological tissue of autogenic, allogenic andxenogenic origin suitable for implantation into humans or animals as surgicaland vascular prostheses. The resultant material inhibits in vivo calcificationand provides a non-porous biomatrix which is impervious to angiogenesis andtissue ingrowth and suitable for the adhesion and retention of transplantedliving cells such as endothelial cells, without the need for additionalextracellular matrix protein coating. The method, which incorporates a gradualincrease in glutaraldehyde concentration from 0% to no more than 5%weight/volume in a pH which is gradually changed from acid to alkaline at roomtemperature, maintains the micro-architecture and the cellular lining of thematerial. Additionally flexibility, compliance and haemo-compatibility alongwith strength and durability can be varied according to the end use. Theglutaraldehyde penetrates the material gradually and completely, cross linkingthe collagenous components of the tissue.
