A method for producing a recombinant glycoprotein under fermentative culture conditions in eukaryotic cells, which affects biomass production and / or N-glycan maturity of the expressed glycoprotein. A method comprising adjusting the concentration of iron, copper, zinc, and manganese in a culture medium during culturing. [Selection diagram] None【課題】バイオマス生成及び／又は発現された糖タンパク質のＮ－グリカン成熟度に影響を与える、真核細胞における発酵培養条件下での組換え糖タンパク質の作製の方法。【解決手段】培養中に、培養培地中の鉄、銅、亜鉛、及びマンガンの各々の濃度を調整する工程を含む、方法。【選択図】なし
