The present disclosure is directed generally towards to embryo insertion systems and methods for use with manufactured seeds. In some embodiments, embryo insertion systems according to the disclosure include an embryo delivery assembly and a fill material deposit assembly. The embryo delivery assembly may include an insertion member, a release assistance member, and a fill material deposit assembly. The fill material deposit assembly may include a fill material containment device, a fill material conveyance device, and optionally, a fill material separation device. Further aspects of the disclosure relate to methods for operating embryo insertion systems according to embodiments of the disclosure to enable simultaneous embryo insertion and fill material delivery.