The present disclosure provides an engineered stem cell, comprising a vector comprising a polynucleotide comprising a nucleic acid sequence of suicide gene, a nucleic acid sequence of immune checkpoint gene and a natural cytotoxicity triggering receptor or a TNF-related apoptosis-inducing ligand, wherein the stem cell is a tumor-targeting cell. The present disclosure also provides a method for treating a cancer or enhancing intratumor immunity or enhancing immunity in tumor microenvironment in a subject, comprising administering an effective amount of the engineered stem cell of the present disclosure to the subject.本揭示內容提供工程化幹細胞，其包含載體，該載體包含含有自殺基因之核酸序列、免疫檢查點基因之核酸序列之聚核苷酸及天然細胞毒性觸發受體或TNF相關之細胞凋亡誘導配體，其中該幹細胞係靶向腫瘤之細胞。本揭示內容亦提供治療個體之癌症或增強腫瘤內免疫性或增強腫瘤微環境中之免疫性之方法，其包含向該個體投與有效量之本揭示內容之工程化幹細胞。
