The invention relates to a high efficient dengue vaccine with biodegradability, a preparing method and a pharmaceutical composition of the same, wherein the high efficient dengue vaccine with biodegradability comprises a charged nano-composite structure with biodegradability. The nano-composite structure comprises a dengue virus protein so that the nano-composite structure can have antibody expression after two vaccinations. Accordingly, comparing to aluminum salt adjuvants and Ribi adjuvants used in prior arts, it can reduce the vaccination times and further lower the immune costs to be a mixed vaccine with commercial grade quality more advantageously.本發明係有關於一種具生物分解之高效率登革疫苗、其製備方法及含彼之醫藥組合物，其中該具生物分解之高效率登革疫苗包括一具生物分解性且帶電性之奈米複合物結構，而於奈米複合物結構中包含一登革病毒蛋白，使奈米複合物結構於兩次之接種後，即具有抗體表現量；藉此，與習知用於登革疫苗之鋁鹽佐劑以及Ribi佐劑相較下，可減少所需接種疫苗的次數，進而降低免疫所需費用，更有利成為具商業等級品質的混合疫苗。
