MARGARET KAROW,SEAN STEVENS,LYNN MACDONALD,Andrew J. MURPHY,George D. YANCOPOULOS,DAVID VALENZUELA,ARIS ECONOMIDES
申请号：
MX2013012216
公开号：
MX343591B
申请日:
2003.08.15
申请国别(地区):
MX
年份:
2016
代理人:
摘要：
A method for engineering and utilizing large DNA vectors to target, via homologous recombination, and modify, in any desirable fashion, endogenous genes and chromosomal loci in eukarytoic cells. These large DNA targeting vectors for eukaryotic cells, termed LTVECs, are derived from fragments of cloned genomic DNA larger than those typically used by other approaches intended to perform homologous targeting in eukaryotic cells. Also provided is a rapid and convenient method of detecting eukaryotic cells in which the LTVEC has correctly targeted and modified the desired endogenous gene(s) or chromosomal locus (loci) as well as the use of these cells to generate organisms bearing the genetic modification.La presente invención se refiere a un ratón transgénico que produce anticuerpos híbridos que contienen regiones variables humanas y regiones constantes de ratón, caracterizado porque el ratón comprende un reemplazo in situ de las regiones VDJ de ratón con regiones VDJ humanas en un locus de cadena pesada de inmunoglobulina murina cromosómica y un reemplazo in situ de regiones VJ de ratón con regiones VJ humanas en un locus de cadena ligera de inmunoglobulina murina cromosómica.
