Disclosed is a system and method for determining the compatibility level of users by creating an emotional DNA profile for the user and matching the emotional DNA profile with profiles of other users. Based on the matching performed, appropriate content or product is displayed to the user or the level of compatibility aspect between individuals is determined. The emotional DNA profile is created by receiving inputs from various sensors that can measure user's physiological responses to content as various signals such as, facial expression, audio tone, biometrics, eyetracking and the like for various time slices and/or optionally sub-segments of standard probe content. Based on the emotional DNA profile created for the user, the overall personality is determined by optionally augmenting additional explicitly mentioned personality information of the user. Further, the emotional DNA profile that is created is matched with other users profile to determine the level of compatibility aspect between individuals.