Usually it cannot utilize the galactose as carbon source, but in order the galactose to utilize as the only carbon source, the low eukaryotic cell which is operated be inherited in accordance with the method in this inventing, for example pichia pasutorisu (Pichia pastoris) it states. The said cell is operated in order to reveal how many of the enzyme which forms ruroaru route, be inherited. Especially, the said cell is operated in order to reveal the galactose permease garakutokinaze, depending upon the UDP galactose C4 epimerase and the galactose 1 phosphoric acid gourd gill transferase and case, be inherited. In addition, in order to produce the glycoprotein which possesses the N glycan which possesses the galactose residue, the enzyme which is operated be inherited, forms ruroaru route usually method in order to improve the yield of the glycoprotein which possesses the galactose terminal N glycan or the galactose content N glycan in the cell which is lacked, is offered. The said method, garakutokinaze, includes the fact that character it converts the said cell with the nuclear acid molecule of 1 or more where the UDP galactose C4 epimerase and the galactose 1 phosphoric acid gourd gill transferase the cord/code it does. It rearranges method and the host cell which are stated and, it makes the existence or lack of the ability to assimilate the galactose as a selective method for producing the cell, possible. As for the said method and the host cell, although the immunoglobulin and the like which, possesses the galactose terminal N glycan or the galactose content N glycan is produced especially being useful is shown in this bill.ガラクトースを炭素源として通常は利用し得ないが、ガラクトースを唯一の炭素源として利用するように本発明における方法に従い遺伝的に操作されている下等真核細胞、例えばピチア・パストリス(Pichia pastoris)を記載する。該細胞は、ルロアール経路を構成する酵素の幾つかを発現するように遺伝的に操作される。特に、該細胞は、ガラクトキナーゼ、UDP-ガラクトース-C4-エピメラーゼおよびガラクトース-1-リン酸ウリジルトランスフェラーゼおよび場合によってはガラクトースパーミアーゼを発現するように遺伝的に操作される。また、ガラクトース残基を有するN-グリカンを有する糖タンパク質を産生するように遺伝的に操作されている、ルロアール経路を構成する酵素を通
