A fundus imaging system is used to generate fundus images when a patient has not received mydriatic pharmaceuticals. The fundus imaging system includes a processor and memory, an infrared light source, and an image sensor array. The infrared light source illuminates the patient's fundus using only infrared light. The fundus imaging system uses one or more different near-infrared wavelengths to illuminate the patient's fundus, and the fundus imaging system captures images during infrared light illumination. Then the fundus imaging system generates a color image of the patient's eye fundus based on generated lightness maps of the infrared-illuminated images.