A blood sample collection assembly (10) comprising: a needle assembly (11) comprising a needle cannula (38) having an intravenous end with a puncture tip (44) with a bevel (74) and a end (46) opposite the patient mounted to a connector (12), the connector (12) extending between a distal end (14) and a proximal end (16) and including an anti-rotation element (64) extending from a outer surface (18) thereof; and a container housing (22) defining a receiving chamber (24) and including a rear end (26) adapted to receive a sample collection tube inside the chamber (24) and a front end (28) that It includes a receiving hole (30) that extends therethrough and configured to receive at least a portion of the connector (12), the receiving hole (30) including a structure corresponding to the anti-rotation element (64) of the connector ( 12) for application with it to prevent rotational movement of the connector (12) with respect to the container housing (22) around a longitudinal axis defining the blood sample collection assembly (10); the connector (12) and the receiving hole (30) including an interconnection structure for axially blocking the connector (12) with the container housing (22), making contact with the anti-rotation element (64) of the connector (12) with the corresponding structure of the receiving hole (30) thereby aligning the connector (12) and the needle cannula (38) to a predetermined position and limiting the rotation of the connector (12) with respect to the container housing (22) around of the longitudinal axis, characterized in that the interconnection structure of the connector (12) includes a locking projection (60) that extends perimetrically around the outer surface (18) of the connector (12), axially locking the connector (12 ) with respect to the container housing (22), and the anti-rotation element (64) cuts and contacts the locking boss (60).Un conjunto (10) de toma de muestra de sangre que comprende: un conjunto de aguja (11) que comprende una c