A container for culture of a plant includes the container body (1) of a substantially airtightness structure in which a culture medium (2) for culture of a plant is placed. A partition wall (3) for vertically partitioning the interior of the container body (1) into two sections and supporting the culture medium (2) is provided in the container body (1), the upper section of the container body (1) partitioned by the partition wall (3) is made as a high humidity chamber (4) and the lower section is made as a low humidity chamber (5). A vent hole (7) for communicating the culture medium (2) with the high humidity chamber (4) is provided in the partition wall (3), the high humidity chamber (4) is brought into a high humidity state, the low humidity chamber (5) is brought into a low humidity state, and then air of a low humidity is supplied to the nutrient medium (2) through the vent hole (7). Consequently, a container for culture of a plant, a method for culture of a plant and a method for production of a cutting seedling, in which culture of a plant can be carried out under desirable humidity conditions and the growth rate or extension of the plant root can be improved, can be obtained.