A population of hematopoietic stem cells expanded obtained by ex vivo culture yprogenitoras hematopoietic stem cells Blood neonatal umbilical cord seeded in medium queproporciona to said stem cells conditions for cell proliferation culture in the presence of tetraethylenepentamine (TEPA), in which said culture medium comprising cytokines early action andin that said TEPA and said proliferation conditions result in (i) increased proliferation of prolonged célulasmadre (Ii) greater long term expansion of clonogenic cells and (iii) maintaining a mayorporcentaje stem cells of cord blood undifferentiated their undifferentiated state encomparación stem and progenitor cells of umbilical cord blood cultured in the presence of dichascondiciones proliferation and absence of TEPA, thus inhibiting the differentiation, while permitting expansion of said population of yprogenitoras hematopoietic stem cells of cord blood in said culture medium, and wherein said hematopoietic stem and progenitor cord blood therefore seexpanden cells but differ more compared to stem cells from umbilical cord blood live sembradasex of it said population of stem cells and hematopoietic progenitor expanded expanded.Una población de células madre hematopoyéticas expandida obtenida por cultivo ex vivo de células madre yprogenitoras hematopoyéticas de sangre del cordón umbilical neonatal sembradas en un medio de cultivo queproporciona a dichas células madre condiciones para proliferación celular y en presencia de tetraetilenpentamina(TEPA), en la que dicho medio de cultivo comprende citocinas de actuación temprana yen la que dicha TEPA y dichas condiciones de proliferación dan como resultado (i) mayor proliferación de célulasmadre prolongada (ii) mayor expansión a largo de plazo de células clonogénicas y (iii) mantenimiento de un mayorporcentaje de células madre de sangre del cordón umbilical indiferenciadas en su estado indiferenciado encomparación con células madre y progenitoras de sangre del cordón um
