An ultrasound diagnostic apparatus and an operating method thereof are disclosed. The operating method includes setting a region of interest (ROI) intended to be enlarged in an ultrasound image of an object, acquiring ultrasound data corresponding to a candidate region including the ROI, and generating a candidate image corresponding to the candidate region. The candidate region may have a larger area than that of the ROI. Shaking of the ultrasound probe is detected by detecting motion of at least one reference pixel in the ultrasound image and/or by sensors such as an acceleration sensor. A portion of the candidate image corresponding to the ROI is displayed, wherein the detected shaking of the probe is corrected.
