A microbial pesticide which is safe for the environment, fast-acting and excellent in an insecticidal effect in case of spray treatment for eggs and larvae of a pest is required. The invention provides a method for controlling at least one pest selected from the group consisting of whiteflies, aphid, spider mites, thrips, rust mites, leaf miners, pyralidae, cabbage moths and longhorn beetles, with Lecanicillium muscarium strain V-5 (deposition number: FERM BP-11135); and a microbial pesticide comprising the strain.對環境友善、速效且在對害蟲之卵、幼蟲進行散佈處理時有效的微生物農藥正被需求著。本發明係使用蠟蚧輪枝菌(Lecanicillium muscarium)V-5菌株(寄存編號:FERM BP-11135),提供可防制由粉蝨類、蚜蟲類、葉蜹、薊馬類、銹蜹類、斑潛蠅、蠹蟲類、夜蛾類及天牛類所組成群選出之至少一種害蟲的方法,以及含有該菌株之微生物農藥。