In the disclosed methods for manufacturing a regenerated tooth unit, regenerated tooth buds can be cultivated in vivo without pressure or space restricting the shape of the teeth, and the extension of the teeth can be controlled. Said methods allow the transplant of regenerated teeth even in cases in which the missing tooth region is large. One disclosed method includes a step in which a regenerated tooth bud is disposed and cultivated inside a spacer in vivo in a mammal. Said spacer is configured so as to be able to: prevent excessive pressure from being applied to the regenerated tooth bud from tissues inside the body of the mammal and prevent the regenerated tooth bud from extending more than a maximum tolerance. Furthermore, said spacer is configured such that the regenerated tooth bud disposed inside can connect with the area outside the spacer. Another disclosed method includes a step in which a plurality of regenerated tooth buds are disposed and cultivated inside a spacer in vivo in a mammal.本發明之課題係提供即使於生體內培養再生牙胚,亦不會因壓力及空間而限制牙齒的形狀,且可控制牙齒伸長之方法。另一課題係即使牙齒缺損部位大型時,亦提供可移植再生牙之方法。本發明係提供一種再生牙單套體之製造方法,其係包含於哺乳動物的生體內,於間隔構件內配置再生牙胚後進行培養的步驟之再生牙單套體的製造方法,該間隔構件係可防止該哺乳動物生體內的組織對該再生牙胚施加過多壓力,及可防止該再生牙胚的伸長超過最大容許值以上之構造,且配置於內部的再生牙胚為可與間隔構件外部連通之構造。另外,本發明係提供包含於哺乳動物的生體內,於間隔構件內配置複數個再生牙胚後進行培養的步驟之再生牙單套體的製造方法。
