KASHYAP, SUBHADRA DEVI,KISHORE, REDDY,VIRAF, SAM VASANIA
申请号：
NZ57074607
公开号：
NZ570746A
申请日:
2007.02.23
申请国别(地区):
NZ
年份:
2012
代理人:
摘要：
Provided is a method for generating a conjunctival tissue system comprising culturing cells of a tissue comprising conjunctival progenitor cells on a support material that is a biocompatible membrane or extracellular matrix in a single medium comprising: (a) Dulbeccos Modified Essential Medium (DMEM), DMEM:F-12 (1:1) medium, or Hams F-12 (1:1) medium (b) a nutrient serum selected from the group consisting of knock-out serum, heat- inactivated human serum, human cord blood serum, human serum albumin, and fetal bovine serum (c) epidermal growth factor (EGF) (d) basic fibroblast growth factor (bFGF) (e) insulin (f) sodium selenite (g) transferrin and (h) an antibiotic or mixture of antibiotics, wherein the single medium supports the proliferation and expansion of the conjunctival progenitor cells on the support material to form a conjunctival tissue system.
