Provided are an anti-CD40 antibody, an antigen binding fragment thereof, and amedical use thereof. Also provided are a chimeric antibody and a humanizedantibodyincluding a CDR region of the anti-CD40 antibody, a pharmaceutical compositionincluding the human anti-CD40 antibody and the antigen binding fragmentthereof,and an application thereof as an anti-cancer drug. In particular, provided areahumanized anti-CD40 antibody, and an application thereof in preparation of adrug totreat a CD40-mediated disease or disorder.