HARRIMAN, WILLIAM DON,ETCHES, ROBERT,LEIGHTON, PHIL
申请号：
EP10808712
公开号：
EP2464220A4
申请日:
2010.08.11
申请国别(地区):
EP
年份:
2014
代理人:
摘要：
A transgenic animal is provided. In certain embodiments, the transgenic animal comprises a genome comprising: an immunoglobulin light chain locus comprising: a) a functional immunoglobulin light chain gene comprising a transcribed variable region encoding: i. light chain CDR1, CDR2 and CDR3 regions that are composed of 2 to 5 different amino acids； and ii. a light chain framework； and, operably linked to the functional immunoglobulin light chain gene: b) a plurality of pseudogene light chain variable regions each encoding: i. light chain CDR1, CDR2 and CDR3 regions that are composed of the same 2 to 5 different amino acids as the CDRs of the functional gene； and ii. a light chain framework that is identical in amino acid sequence to the light chain framework of the transcribed variable region.
