The present invention provides nano-sized particles of melanin, and the method for formation of the melanin particles comprise the following steps: adding a base to a dopamine.H+X−-containing solution (wherein H+X− is an acid) and allowing an acid-base neutralization reaction and forming nano-sized particles of melanin by controlling the addition of nucleic dopamine.H+X− (a) to base (b) at a ratio of a:b=1:0.1-1, and allow concurrent or consecutive formation of melanin by oxidation curing of the dopamine in air (polymerization). The manufacturing method according to the present invention can produce nano-sized melanin particles in a short period of time. Furthermore, the nano-sized melanin particles made according to the present invention are distinctive from conventional solvents containing dispersions of natural melanin and synthetic melanin, and have excellent application in various fields.