A solid medium of Antrodia cinnamomea comprises 20 to 50 wt % soybean 30 to 50 wt % coixseed and 20 to 50 wt % sorghum. A culturing method of Antrodia cinnamomea, comprises providing a solid medium comprising soybean, coixseed and sorghum preparing a solution of Antrodia cinnamomea, inoculating the solution of Antrodia cinnamomea on the solid medium in a concentration of 1×103 to 1×106 cfu on per 100 g of solid medium, and statically culturing the solution of Antrodia cinnamomea on the solid medium till obtaining hyphae adhered on the solid medium and culturing the solution of Antrodia cinnamomea by intermittent shaking in a frequency of every 5 to 7 days shaking 5 to 10 minutes, with a period of the culturing by intermittent shaking being more than 40 days.
