A drill guide for an anterior cruciate ligament reconstruction operation is provided. The drill guide allows a transplantation hole on a tibial bone and a transplantation hole on a femoral bone to be properly positioned without relying upon the experience and sense of the operator. The drill guide for an anterior cruciate ligament reconstruction operation includes a guide member regulating a movement of a drill guide in a direction perpendicular to an axis of the same, a pointing member having a tip part located in a position on an imaginary line extending from an axis of the guide member, and a light beam radiating member radiating a light beam traveling along the imaginary line extending from the axis of the guide member.