A photoacoustic wave measurement instrument includes a light output unit, a photoacoustic wave detection unit and a photoacoustic wave generation member. The light output unit outputs light. The photoacoustic wave detection unit receives a photoacoustic wave generated by the light in a measurement object, and converts the light into an electric signal. The photoacoustic wave generation member is arranged between an light output end of the light output unit and the measurement object, receives the light, and generates a known photoacoustic wave.