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FREMGANGSMÅDE TIL IDENTIFIKATION AF APTAMERER
专利权人:
Albert-Ludwigs-Universität Freiburg
发明人:
ROTH, Günther,STREHLITZ, Beate,REINEMANN, Christine
申请号:
DK20120780712T
公开号:
DK2771485(T3)
申请日:
2012.10.25
申请国别(地区):
丹麦
年份:
2018
代理人:
摘要:
The invention relates to a method for identifying aptamers, having the following steps —bringing a mixture of oligonucleotides into contact with an aptamer target structure and binding at least some of the oligonucleotides to the target structure, —separating the oligonucleotides which have been bound to the aptamer target structure from the aptamer target structure and from oligonucleotides that are not bound to the aptamer target structure, —amplifying individual oligonucleotides which were bound to the aptamer target structure in a physically separate manner and producing a plurality of physically separate amplicons, each amplicon predominantly containing one type of oligonucleotides, —specifying a specific marker for a plurality of the physically separate amplicons such that each of the marked amplicons can be uniquely identified using the specified marker of the amplicon, —sequencing oligonucleotides in a plurality of marked amplicons and assigning the marker that is specific for the amplicon t
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