DAOUST, Marc-Andre,LANDRY, Nathalie,LAVOIE, Pierre-Olivier,ARAI, Masaaki,ASAHARA, Naomi,MUTEPFA, David Levi Rutendo,HITZEROTH, Inga Isabel,RYBICKI, Edward Peter
申请号：
CACA2013/050364
公开号：
WO2013/166609A1
申请日:
2013.05.10
申请国别(地区):
WO
年份:
2013
代理人:
摘要：
A method of producing a virus-like particle (VLP) in a plant is provided. The method comprises introducing a first nucleic acid into the plant, or portion of the plant. The first nucleic acid comprising a first regulatory region active in the plant operatively linked to a nucleotide sequence encoding one or more rotavirus structural protein for example but not limited to rotavirus protein VP2. The nucleotide sequence may further comprise one or more than one amplification element and/or a compartment targeting sequence. A second nucleic acid might be introduced into the plant, or portion of the plant. The second nucleic acid comprises a second regulatory region active in the plant and operatively linked to a nucleotide sequence encoding one or more rotavirus structural protein, for example but not limited to rotavirus protein VP6. Optionally, a third nucleic acid and/ or fourth nucleic acid might be introduced into the plant, or portion of the plant. The third nucleic acid comprises a third regulatory region active in the plant and operatively linked to a nucleotide sequence encoding one or more rotavirus structural protein, for example but not limited to rotavirus protein VP4. The fourth nucleic acid comprises a fourth regulatory region active in the plant and operatively linked to a nucleotide sequence encoding one or more rotavirus structural protein, for example but not limited to rotavirus protein VP7. The plant or portion of the plant is incubated under conditions that permit the expression of the nucleic acids, thereby producing the VLP.Linvention concerne un procédé pour produire une particule de type virus (VLP) chez une plante consistant à introduire un premier acide nucléique dans la plante ou dans une partie de cette dernière. Le premier acide nucléique comprend une première région régulatrice active dans la plante fonctionnellement liée à une séquence nucléotidique codant pour une ou plusieurs protéine(s) structurelle(s) de rotavirus, par exemple de faç
