Muhammad Fahim,Philip John Larkin,Ligia Isabel Ayala-Navarrete,Anthony Alan Millar
申请号:
US12807969
公开号:
US08536403B2
申请日:
2010.09.17
申请国别(地区):
US
年份:
2013
代理人:
摘要:
The present invention provides a transgenic wheat cell or wheat plant, the wheat cell or wheat plant comprising a chimeric DNA molecule which encodes a dsRNA molecule which is capable of inhibiting wheat streak mosaic virus (WSMV) replication, wherein the wheat cell or plant is immune to WSMV. The present invention also provides a chimeric DNA, the chimeric DNA comprising (i) a wheat expressible promoter (ii) a region which encodes a dsRNA which is capable of inhibiting WSMV replication and (iii) a transcription termination and polyadenylation signal. Finally, the present invention provides a process for producing the aforementioned transgenic wheat cell or plant, comprising (i) introducing a chimeric DNA molecule comprising (a) a wheat expressible promoter (b) a region which encodes a dsRNA which is capable of inhibiting WSMV replication and (c) a transcription termination and polyadenylation signal into a parental wheat cell and optionally (ii) regenerating a wheat plant from the wheat cell comprising the chimeric DNA molecule and (iii) identifying and/or selecting a plant which is immune to WSMV.