A handheld biopsy punch pen assembly with a first sampling housing selectively removably couplable to a body of the assembly and having a skin puncturing assembly disposed therein that includes a distal tip and with at least one blade member disposed thereon. The assembly includes a spring-loaded pen assembly operably configured to have a loaded position with the distal tip of the skin puncturing assembly recessed, a sampling position with the distal tip projecting outwardly away from the lower end of the first sampling housing for sampling of a user's skin and with the distal end the plunger member directly coupled to the skin puncturing assembly, and a retracted position with the distal tip projecting outwardly away from, and disposed a length away from, the lower end of the first sampling housing and with the distal end the plunger member uncoupled to the skin puncturing assembly.
