The present invention is in the field of plant genetics and provides recombinant nucleic acid molecules, constructs, and other agents associated with the coordinate manipulation of multiple genes in the fatty acid synthesis pathway. In particular, the agents of the present invention are associated with the simultaneous enhanced expression of certain genes in the fatty acid synthesis pathway and suppressed expression of certain other genes in the same pathway. Also provided are plants incorporating such agents, and in particular plants incorporating such constructs where the plants exhibit altered seed oil compositions, e.g. soybean plants bearing seed, wherein the seed exhibit an oil composition which comprises 42-85% oleic acid and 0.5-8% saturated fatty acids.The genes to be suppressed are selected from the group consisting of FAD2, FAD3, and FATB, and the genes whose expression is to be enhanced are selected from the group consisting of beta-ketoacyl-ACP synthase I, beta-ketoacyl-ACP synthase IV, and delta-9 desaturase.
