An arthroscopic positioning instrument is provided to overcome the problem of inconvenient operation of the modern arthroscopic positioning instrument. The arthroscopic positioning instrument includes a handle, a positioning member and a guiding cylinder is disclosed. The handle includes a finger holding member, a palm holding member and at least one connecting member. The palm holding member is connected to the finger holding member via the at least one connecting member. The finger holding member includes one end provided with an engaging portion and an arched guiding groove. The palm holding member does not intrude into a maximum axial range of the engaging portion. The positioning member includes an arched end and a hook end. The arched end is received in the arched guiding groove. The guiding cylinder is coupled with the engaging portion of the finger holding member.