LIPPMAN, Zachary,RODRIGUEZ-LEAL, Daniel,JACKSON, David
申请号：
WO2018US33143
公开号：
WO2018213547(A1)
申请日:
2018.05.17
申请国别(地区):
世界知识产权组织国际局
年份:
2018
代理人:
摘要：
Provided herein are compositions and methods for generating alleles of genes of interest in plants. In some aspects, libraries of plants or seeds are provided that comprise an expression construct comprising a RNA-guided endonuclease (e.g., a Cas9 endonuclease) and multiple different guide RNAs that target regions of the gene of interest, such as regulatory regions.
