A lens holder (104) for holding a vitrectomy lens (102) during a surgical procedure, comprising: a support ring (120) sized and shaped to receive a portion of a vitrectomy lens to provide visualization of a surgical site in an eye of a patient, the support ring being configured to rest upon the eye of the patient during a surgical treatment procedure performed on the eye； a transparent, flexible membrane (124) disposed along a bottom plane of the support ring, and configured to conform to a surface feature on the eye and to separate a wetting agent in the support ring from the eye. The support ring comprises an interior surface (130), an exterior surface (132), and at least one perforation (134) extending through a side of the support ring from the interior surface to the exterior surface. The lens holder comprises and an overflow wall (122) that extends from the lower edge (138) of the support ring in an upward direction tapering outwardly from the support ring so as to form an overflow trough (154) that extends about the ring.
