A glove dispensing assembly includes a stack of interfolded gloves. In particular, the gloves are folded in an S-like arrangement having at least two folds per glove. The glove dispensing assembly additionally may include a soft, collapsible dispenser made of an elastomeric material to contain the stack of gloves. A method of assembling the glove dispensing assembly with the dispenser containing the stack of interfolded gloves is also provided.