CIGAN ANDREW MARK,GAO HUIRONG,LIU ZHAN BIN,MUTTI JASDEEP,PODLICH DEAN,SCELONGE CHRISTOPHER
申请号:
IN201717004288
公开号:
IN201717004288A
申请日:
2017.02.06
申请国别(地区):
IN
年份:
2017
代理人:
摘要:
Compositions and methods are provided for introducing transgenic target sites for Site Specific Integration (SSI) and/or polynucleotides of interest into at least one double strand break target site of a double strand break inducing agent in a genomic window of a plant genome. Also provided are methods and compositions for producing a complex trait locus in a genomic window of a plant comprising at least one transgenic target site for site specific integration integrated in at least double strand break target site. The double strand break target site can be but is not limited to a target site for a zinc finger endonuclease an engineered endonuclease a meganuclease a TALENs and/or a Cas endonuclease. The genomic window of said plant can comprise at least one genomic locus of interest such as a trait cassette a transgene a mutated gene a native gene an edited gene or a site specific integration (SSI) target site.