NEW ZEALAND FOREST RESEARCH INSTITUTE LIMITED;WALTER, CHRISTIAN;SMITH, DALE, RAYMOND
发明人:
WALTER, CHRISTIAN,SMITH, DALE, RAYMOND
申请号：
WO1996NZ00062
公开号：
WO9701641(A1)
申请日:
1996.06.25
申请国别(地区):
世界知识产权组织国际局
年份:
1997
代理人:
摘要：
The invention provides a technique for stably inserting foreign genes into conifer cells. The cells to be transformed are in an undifferentiated state such that embryos are likely to regenerate from a single cell. Because of the use of this approach, the probability that the stably transformed plants will be chimaeric is low or non-existent. Conifer embryogenic tissue may be transformed subjected to extremely toxic levels of selection agent after insertion of foreign genes wherein the foreign genes include genes protective against the selection agent and wherein only stably transformed embryogenic tissue grows on the medium, and non-transformed tissue does not grow. The selected tissue may be used to produce transgenic plants. In preferred embodiments the cells to be transformed are maintained on a medium having concentrations of inorganic ions within a prescribed range.
