GOODYEAR, MARK DAVIS,HUETHER, MICHAEL JOHN,MANNAN, RAMASAMY MANNAR,OIEN, NANCEE LOIS
申请号：
NZ59574807
公开号：
NZ595748A
申请日:
2007.08.30
申请国别(地区):
NZ
年份:
2013
代理人:
摘要：
Patent No. 595748 Disclosed is a method of preparing a heat treated Leptospira bacterin, comprising the steps of: (a) measuring the lipase activity of the bacterin； (b) heating the bacterin to a temperature of 60 to 70°C for 5 to 10 hours； (c) measuring the lipase activity of the bacterin after heat treatment； (d) comparing the lipase activity of the bacterin before heating to the lipase activity of the bacterin after heating； and (e) selecting a heat treated bacterin wherein the lipase activity after heat treatment is 50% or less of the lipase activity of the bacterin before heat treatment, wherein said bacterin comprises a suspension of killed Leptospira bacteria having acceptable antigenic activity. Also disclosed is an emulsion vaccine comprising heat treated Leptospira canicola, Leptospira grippotyposa, Leptospira hardjo, Leptospira icterohaemorrhagiae, and Leptospira pomona bacterins obtained by heating the bacterins to a temperature of 65°C for 8 hours, and further comprising AMPHIGEN®, adjuvants, preservatives, and diluting buffer so that the concentration of the bacterins are at 1200 NU/5ml dose for all Leptospira bacterins except Leptospira hardjo, which is at 2400 NU/5ml dose, wherein the emulsion vaccine is homogenized and microfluidized, and then the pH adjusted to pH 7.0-7.3.
